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Plant Tissue Culture: Techniques and Experiments 4th Edition by Sunghun Park, ISBN-13: 978-0128211205

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Plant Tissue Culture: Techniques and Experiments 4th Edition by Sunghun Park, ISBN-13: 978-0128211205

[PDF eBook eTextbook]

  • Publisher: ‎ Academic Press; 4th edition (March 5, 2021)
  • Language: ‎ English
  • 252 pages
  • ISBN-10: ‎ 0128211202
  • ISBN-13: ‎ 978-0128211205

Teaches users how to master the key concepts of plant tissue culture using step-by-step protocols.

Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant culturing for almost 30 years. The book’s experiments demonstrate major concepts and can be conducted with a variety of plant materials readily available throughout the year. This fully updated edition describes the principles of the newest technologies, including CRISPR/Cas9 gene editing and RNAi technology with plant cell and tissue cultures and their applications. Bridging the gap between theory and practice, this book contains detailed methodology supported by comprehensive illustrations, giving users a diverse learning experience for both university students and plant scientists.

Plant cell, tissue and organ culture is an important research tool in both basic and applied studies as well as in commercial applications.

Table of Contents:

1. History of plant tissue culture 2. Setup of a tissue culture laboratory 3. Media components and preparation 4. Explant preparation 5. Contamination 6. Callus induction 7. Regeneration and morphogenesis 8. Woody shrubs and trees 9. Haploid plants from anther culture 10. Embryo rescue 11. Meristem culture for virus-free plants 12. In Vitro propagation for commercial production of ornamentals 13. Protoplast isolation and fusion 14. Agrobacterium-mediated transformation of plants 15. Genome edition and RNAi

Sunghun Park is Professor at the Department of Horticulture and Natural Resources at Kansas State University, USA. He earned his PhD in Plant Physiology from Texas A&M University in 1995 and his main research areas include abiotic stress physiology, crop improvement using plant tissue culture and genome editing technology.

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